AMC Vaginal - Complete Analysis of the Genital Tract

Description

What is AMC Vaginal?

It is the complete study of all the microorganisms that colonize the vagina and make up an entire ecosystem in the female genital tract. The quantity and type of bacteria and other microorganisms present have significant implications for a woman’s overall health and her reproductive capacity.

The analysis enables the identification of all the microorganisms present in the vaginal microbiota and the relationships between them, determining whether there is dysbiosis and identifying pathogenic microorganisms.

What Does It Identify?

• All microorganisms of the vaginal microbiota
• Microbial relationships and ecosystem
• Vaginal dysbiosis and imbalances
• Pathogenic microorganisms

Sexually Transmitted Infections Detected

1

Chlamydia

Chlamydia trachomatis

2

Gonorrhea

Neisseria gonorrhoeae

3

Candidiasis

Vaginal fungal infections

4

HIV

Human immunodeficiency virus

5

HPV

Human papillomavirus - Different genotypes

Advanced NGS Technology

We use next-generation sequencing (NGS) to obtain accurate and complete results of the vaginal ecosystem in 15 business days.

Technology

Advanced Analysis Technology

Specialized NGS Sequencing

We employ NGS (Next Generation Sequencing) technology optimized for the analysis of the complete vaginal microbial ecosystem, including bacteria, fungi, viruses and other microorganisms.

• Complete identification: All microorganisms present
• Quantitative analysis: Relative abundance of each species
• Microbial relationships: Ecosystem interactions
• Pathogen detection: Disease-causing microorganisms

Detection of Sexually Transmitted Infections

Our analysis includes the specific detection of the main STIs using high-precision molecular techniques.

• Bacterial: Chlamydia, Gonorrhea
• Viral: HIV, HPV (genotypes)
• Fungal: Vaginal candidiasis
• HPV genotyping: Strain identification
• Resistances: Antimicrobial profiles
• Viral load: Quantification when applicable

Vaginal Dysbiosis Assessment

We analyze the balance of the vaginal ecosystem to detect alterations that may affect a woman’s reproductive and overall health.

• Dominant lactobacilli: L. crispatus, L. gasseri, L. iners
• Pathogenic species: Gardnerella, Atopobium, Prevotella
• Diversity indices: Shannon, Simpson
• Predictive vaginal pH: Microbiota-based estimation

Analysis Process

1

DNA extraction

Optimized protocol for vaginal samples

2

Targeted amplification

Specific PCR for vaginal microbiota

3

NGS sequencing

High-depth analysis

4

Bioinformatic analysis

Identification and quantification

5

Clinical report

Gynecological interpretation

Certifications

• CE-IVD certification
• Clinically validated protocol
• ISO 15189 standards
• STI-specific quality control

Sample

Vaginal Sample Collection Protocol

Sample Type

Vaginal smear: Primary sample

Swab: Upper part of the vagina

Self-collection: Available for the patient

Container: Sterile tube with stabilizer

Storage Conditions

• Immediate: Process within 4 hours at room temperature
• Refrigeration: 2-8°C up to 48 hours
• Freezing: -20°C up to 2 weeks
• Stabilizer: With included buffer up to 30 days

Patient Instructions

Prior preparation (24-48 hours before):

• Do not use vaginal douches
• Avoid vaginal pessaries or creams
• Do not have sexual intercourse
• Stop antibiotics (if possible)

Day of collection:

• Avoid being during the menstrual period
• Normal external genital hygiene
• Do not use internal soaps
• Use the self-collection kit provided

Factors That May Affect

• Active menstruation (interference with analysis)
• Systemic antibiotics (up to 2 weeks after)
• Vaginal antifungal treatments
• Recent vaginal douches (48 hours)
• Sexual intercourse (24 hours before)
• Acute vaginal infections being treated

Results

Interpretation of Results

Normal Vaginal Microbiota

Dominant lactobacilli: L. crispatus, L. gasseri, L. iners

Acidic pH: Maintenance of vaginal pH

Low diversity: Stable ecosystem

Absence of pathogens: No STIs detected

Microbial balance: Vaginal eubiosis

Dysbiosis and Pathogens

Bacterial vaginosis: Gardnerella, Atopobium

STIs detected: Chlamydia, Gonorrhea, HPV, HIV

Candidiasis: Candida species

High diversity: Loss of lactobacilli

Elevated pH: Alteration of the environment

Results Timeline

Day 0

Receipt and initial processing

Days 1-10

NGS sequencing and STI analysis

Day 12

Gynecological report available

Clinical Interpretation

• Results should be correlated with clinical symptoms
• The presence of pathogens requires medical evaluation
• Dysbiosis may precede evident symptoms
• Detected STIs require treatment and follow-up
• We recommend consultation with a specialist gynecologist

Clinical indications

Gynecological Indications

• Recurrent bacterial vaginosis Repeated episodes of dysbiosis
• Recurrent vaginal candidiasis Frequent fungal infections
• Nonspecific vaginal symptoms Discharge, itching, irritation without a clear cause
• Post-treatment control Verification of cure

STI Screening

• Routine STI screening
• New sexual partner
• Unprotected sexual intercourse
• Symptoms suggestive of STIs
• Periodic control in at-risk populations

Reproductive Health

• Infertility of unknown cause
• Recurrent miscarriages
• Pregnancy planning
• Assisted reproduction techniques
• Specialized prenatal care

Specialties That Request It

Gynecology

Obstetrics

Microbiology

Internal Medicine

Family Medicine

Clinical Inf. Diseases

Advantages of AMC Vaginal

• Complete ecosystem analysis
• Simultaneous STI detection
• Dysbiosis assessment
• Self-collection available
• HPV genotyping
• Results in 15 days