Oral Microbiota Test - Complete Analysis

Description

What is the Oral Microbiota Test?

It is the complete study of the oral microbiota, that is, all the microorganisms that inhabit the different niches of the oral cavity. The oral cavity can be divided into several zones, each with a specific predominance of microorganisms.

In our test we can detect all possible microorganisms in the oral microbiota: G+ and G- bacteria, archaea, fungi and viruses, all in a single comprehensive test.

Complete Detection

• Gram-positive bacteria (G+)
• Gram-negative bacteria (G-)
• Archaea (primitive microorganisms)
• Fungi (yeasts and filamentous)
• Viruses (DNA and RNA)

Oral Cavity Zones Analyzed

1

Buccal Mucosa

Inner lining of cheeks and lips

2

Dental Surface

Enamel and dental biofilm

3

Gingival Sulcus

Space between tooth and gum

4

Tongue and Palate

Specialized mucosal surfaces

5

Saliva

Suspended microorganisms

Advanced Technology

We use third-generation NGS sequencing with read lengths greater than 4 Mb to achieve maximum precision in the identification of microorganisms.

Technology

Advanced Sequencing Technology

Third-Generation NGS Sequencing

We employ advanced NGS technology that enables long reads greater than 4 Mb, facilitating the complete reassembly of microbial genomes with a lower error rate.

• High quality and precision in genomic sequencing
• Very long read lengths (>4 Mb)
• Easy reassembly of microbial genomes
• Lower error rate compared to previous technologies

Comprehensive Detection of Microorganisms

Our analysis identifies all types of microorganisms present in the oral cavity through broad-spectrum sequencing.

• Gram-positive bacteria: Streptococcus, Enterococcus
• Gram-negative bacteria: Porphyromonas, Fusobacterium
• Archaea: Methanobrevibacter
• Fungi: Candida, Aspergillus
• DNA viruses: Herpesvirus, Papillomavirus
• RNA viruses: Respiratory viruses

High Specificity and Sensitivity

We achieve superior levels of specificity and sensitivity compared to traditional culture methods, enabling the detection of hard-to-grow microorganisms.

• High specificity: Accurate identification of species
• Superior sensitivity: Detection of low microbial load
• Non-culturable microorganisms: Direct detection by DNA
• Resistance profiles: Antimicrobial resistance genes

Technical Workflow

1

DNA extraction

Optimized protocol for oral microbiota

2

Library preparation

Optimization for long reads

3

NGS sequencing

Third-generation technology

4

Bioinformatic analysis

Identification and quantification

5

Clinical report

Specialized interpretation

Certifications

• CE-IVD certification
• Clinically validated protocol
• ISO 15189 standards
• Rigorous quality control

Sample

Oral Sample Collection Protocol

Sample Type

Saliva: Primary non-invasive sample

Buccal swab: Mucosa and gums

Volume: 2-5 ml of saliva

Container: Sterile tube with stabilizer

Storage Conditions

• Immediate: Process within 2 hours at room temperature
• Refrigeration: 2-8°C up to 24 hours
• Freezing: -20°C up to 1 week
• Stabilizer: With included buffer up to 30 days

Patient Instructions

Prior preparation (12 hours before):

• Do not use antimicrobial mouthwashes
• Avoid antibiotics (if possible)
• Maintain usual oral hygiene
• Do not smoke 2 hours before

Day of collection:

• First morning saliva sample
• Do not eat or drink 1 hour before
• Do not brush your teeth beforehand
• Use the collection kit provided

Factors That May Affect

• Systemic antibiotics (up to 2 weeks after)
• Antimicrobial mouthwashes (24-48 hours)
• Recent dental treatments (1 week)
• Active acute oral infections
• Tobacco and alcohol (2 hours before)

Results

Interpretation of Results

Complete Microbial Analysis

G+ bacteria: Streptococcus, Enterococcus, Lactobacillus

G- bacteria: Porphyromonas, Fusobacterium, Prevotella

Archaea: Methanobrevibacter oralis

Fungi: Candida spp., Aspergillus

Viruses: Herpesvirus, Papillomavirus

Parameters Analyzed

Relative abundance: Percentage of each microorganism

Microbial diversity: Shannon and Simpson indices

Oral pathogens: Periodontal bacteria

Antimicrobial resistance: Resistance genes

Virulence factors: Pathogenicity genes

Results Timeline

Day 0

Receipt and initial processing

Days 1-10

NGS sequencing and analysis

Day 12

Clinical report available

Clinical Interpretation

• Results should be correlated with the oral clinical history
• The presence of pathogens does not always indicate active disease
• Microbial diversity is indicative of oral health
• Imbalances may precede clinical symptoms
• We recommend consultation with a periodontics specialist

Clinical indications

Main Oral Pathologies

• Periodontal disease Gingivitis and periodontitis
• Dental caries Cariogenic activity and risk
• Halitosis Bad breath of oral origin
• Fungal infections Oral candidiasis
• Viral lesions Oral herpes, leukoplakia

Systemic Connections

• Cardiovascular disease
• Diabetes mellitus
• Respiratory disease
• Pregnancy complications
• Rheumatoid arthritis

Specialties That Request It

Dentistry

Periodontics

Oral Pathology

Microbiology

Internal Medicine

Pediatrics

Advantages of the Oral Test

• Detection of all microorganisms
• Identification of resistances
• Non-invasive method
• Higher sensitivity than cultures
• Analysis across multiple niches
• Results in 15 days